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TA cloning
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TA cloning : ウィキペディア英語版
TA cloning
TA cloning is a subcloning technique that avoids the use of restriction enzymes〔(【引用サイトリンク】title=Improved TA Cloning )〕 and is easier and quicker than traditional subcloning. The technique relies on the ability of adenine (A) and thymine (T) (complementary basepairs) on different DNA fragments to hybridize and, in the presence of ligase, become ligated together. PCR products are usually amplified using Taq DNA polymerase which preferentially adds an adenine to the 3' end of the product. Such PCR amplified inserts are cloned into linearized vectors that have complementary 3' thymine overhangs.
==Procedure==


抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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